- Why are pure cultures required in the laboratory?
- What are the components of a culture medium indicate their uses?
- What are the basic components of culture media?
- Which of the following are functions of water in the culture medium?
- What are the components of bacterial culture media?
- What is a biological example?
- What is a peptone and how is it prepared from raw materials?
Why are pure cultures required in the laboratory?
As mentioned in the previous lab, pure cultures are essential for microbiological studies. Obtaining a pure culture of bacteria is usually accomplished by spreading bacteria on the surface of a solid medium so that a single cell occupies an isolated portion of the agar surface.
What are the components of a culture medium indicate their uses?
1 Nutrients: proteins/peptides/amino-acids. 2 Energy: carbohydrates. 3 Essential metals and minerals: calcium, magnesium, iron, trace metals: phosphates, sulphates etc. 4 Buffering agents: phosphates, acetates etc.
What are the basic components of culture media?
Components of culture media:
- Water– Source of hydrogen and oxygen.
- Electrolytes– NaCl & other electrolytes.
- Peptone– Mixture of partially digested proteins (animal or vegetable).
- Meat extract, yeast extract – Protein degradation products/carbohydrates/Inorganic salts/Growth factors.
- Blood– It enriches media.
Which of the following are functions of water in the culture medium?
Answer: Water is used in many steps of the tissue or cell culture process. It is the main component of buffers and media, it is used for the dissolution of additives and drugs, and for rinsing bioreactors, plasticware and glassware. Thus, water quality may play an important role in cell culture experimental outcomes.
What are the components of bacterial culture media?
Some of the ingredients of culture media include water, agar, peptone, casein hydrolysate, meat extract, yeast extract and malt extract. Classification: Bacterial culture media can be classified in at least three ways; Based on consistency, based on nutritional component and based on its functional use.
What is a biological example?
The definition of biological is something that relates to life or living. An example of biological is water helping the kidneys flush waste and toxins from the body. An example of biological is a mother and her son to whom she gave birth.
What is a peptone and how is it prepared from raw materials?
Peptones are a mixture of water soluble polypeptides, peptides, amino acids and other substances remaining after the digestion of protein material. Raw materials must stored in a way that prevents the growth of spoilage organisms. Peptones are used in a wide range of microbiological applications.