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What is being tested in Hopkins Cole test?

Hopkin’s Cole test is a specific test used for the detection of indole ring and thus, tryptophan in proteins. The test detects the indole ring that is found in tryptophan amino acid, which in turn helps in the identification of proteins containing tryptophan. …

What is the role of H2SO4 in the Hopkins Cole reagent test?

Concentrated H2SO4 causes dehydration of tryptophan which reacts with Hopkins Cole reagent to give a reddish violet ring between two layers.

What group amino acid present in a protein is identified by Hopkins Cole test?


What is the principle of Sakaguchi test?

Principle of Sakaguchi test: The arginine reacts with α – napththol and an oxidizing agent such as bromine water or sodium hypochlorite/sodium hypobromite to give a red colored product. The other guanidinium containing compounds other than amino acid also give this reaction.

How can I memorize food tests?

Benedict’s solution – detects glucose – blue to brick red. iodine – detects starch – orange to blue black. Sudan 3 – detects lipids – red stained oil layer will separate if lipids are present.

Which food items that you eat have more protein?

Protein foods

  • lean meats – beef, lamb, veal, pork, kangaroo.
  • poultry – chicken, turkey, duck, emu, goose, bush birds.
  • fish and seafood – fish, prawns, crab, lobster, mussels, oysters, scallops, clams.
  • eggs.
  • dairy products – milk, yoghurt (especially Greek yoghurt), cheese (especially cottage cheese)

What is the cleanest meat to consume?

Here are five healthy meats that won’t wreak havoc on your colon.

  1. Buffalo (Bison) No matter how good white meat can be, it will never truly satiate the craving for red meat.
  2. Pork. Pork chops used to be on the doctors’ hit list.
  3. Chicken. White meat is much better for you than red — that’s a well-known fact.
  4. Turkey.
  5. Fish.

Is fish healthier than meat?

Non-fried fish and shellfish such as shrimp, crab and lobster are lower in saturated fat and a healthy alternative to many cuts of meat. Choose fish high in omega-3 fatty acids such as mackerel, lake trout, herring, sardines, albacore tuna and salmon.

Why no meat during Lent but fish is OK?

Fish, Fridays & Lent It simply meant abstaining from eating the flesh of warm-blooded animals—since the thinking goes, Jesus was a warm-blooded animal. Fish, though, which are cold blooded were considered okay to eat on fasting days.

Is fish healthier than chicken?

While the quantity also plays a major role, chicken breast is considered the best for weight loss, while boiled chicken and chicken soups with less salt also helps. Chicken can only be considered slightly less than fish only on the basis of omega-3 fatty acids content in fish.

Do fish suffer when they are dying?

Fish out of water are unable to breathe, and they slowly suffocate and die. Just as drowning is painful for humans, this experience is most likely painful for fish. Just as drowning is painful for humans, this experience is most likely painful for fish.